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Updated: Jan 23, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
SPINK3-Sperm Interaction Determines a Stable Sperm Subpopulation With Intact CatSper Channel
Anabella R Nicolli1, Xiaofang Huang2, Lucia Zalazar1
1Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas (IIB-FCEyN/CONICET), Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mar del Plata, Buenos Aires, Argentina.
Abstract:
Sperm capacitation involves proteolytic remodeling of membrane proteins, including components of the CatSper calcium channel, which is essential for hyperactivation and male fertility. Here, we identify the seminal protease inhibitor SPINK3, a known decapacitation factor that suppresses premature capacitation in the female tract, as the first physiological inhibitor of CATSPER1 processing. In mouse sperm, SPINK3 blocks capacitation-induced CATSPER1 cleavage, preserving a subpopulation with intact CatSper channels and lacking pTyr development in the flagellum. SPINK3 localizes to the outer surface of the sperm principal piece membrane in a CatSper-dependent but non-quadrilateral pattern, stabilizes membrane organization, and delays cholesterol efflux. These results reveal SPINK3 as a multifunctional regulator of capacitation, shaping sperm subpopulations in the female reproductive tract.
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