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Updated: Jan 25, 2026

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
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Protocol to study the role of endogenously produced itaconate using CRISPR-Cas9 technology in THP-1 cells

Luke A Bourner1, Katie A Acken1, Haiyan Long1

  • 1Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, USA.

STAR Protocols
|January 23, 2026
PubMed
Abstract

Keywords:
CRISPRImmunologyMetabolism

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