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Related Experiment Video

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4D Printed Bifurcated Stents with Kirigami-Inspired Structures

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4D-printed ELISA needle panel meter.

Hsiao-Chu Chiu¹, Cheng-Kuan Su¹

¹Department of Chemistry, National Chung Hsing University, Taichung City, 402227, Taiwan, ROC.

Biosensors & Bioelectronics

|January 23, 2026

Summary

This summary is machine-generated.

A novel sensor using digital light processing four-dimensional printing (4DP) enables on-site detection of chloramphenicol (CAP) antibiotics. This redox-responsive device offers a chromogenic-free method for food safety analysis.

Keywords:

Chloramphenicol Enzyme-linked immunosorbent assay Food chemistry Four-dimensional printing Redox-responsive materials

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Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Enzyme-linked immunosorbent assay (ELISA) is crucial for detecting antibiotics in food.
Current ELISA methods often require specialized equipment and lack on-site applicability.
Need for rapid, on-site antibiotic detection methods in food safety.

Purpose of the Study:

To develop an on-site, chromogenic-free ELISA for chloramphenicol (CAP) detection.
To utilize digital light processing four-dimensional printing (4DP) for fabricating a novel sensing device.
To create a redox-responsive sensor for quantitative antibiotic analysis.

Main Methods:

Fabrication of a panel meter with a redox-responsive needle using 4DP and EDT-incorporated resins.
Optimization of anti-CAP antibody coating for competitive ELISA.
Utilizing the competition between CAP and CAP-HRP to induce needle bending dependent on CAP concentration.

Main Results:

The sensor exhibited hydrogen peroxide-dependent bending due to redox-responsive swelling.
Achieved a method detection limit of 2.5 pg mL⁻¹ for CAP.
Validated the method across various food matrices (salmon, shrimp, meat, honey, milk) against conventional ELISA.

Conclusions:

4DP technology can fabricate smart redox-responsive sensing devices.
The developed method offers a reliable and applicable approach for on-site quantitative chemical analyses.
This advancement enhances conventional immunochemical assays for improved food safety monitoring.