Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

33.1K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.1K
DNA Base Pairing02:27

DNA Base Pairing

32.1K
32.1K
DNA Helicases00:55

DNA Helicases

24.0K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.0K
The DNA Helix01:16

The DNA Helix

156.3K
Overview
156.3K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.6K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.6K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

91.0K
91.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enantioselective transplacental transfer of p-phenylenediamine quinones.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

A Guanine-Enhanced Graphene-DNA Paper-Based Sensing Platform Enabling Sensitive Hg<sup>2+</sup> Detection.

Biosensors·2026
Same author

Corrigendum to 'Research progress of single molecule sensors based on DNA platform' [Talanta 302 (2026) 129449].

Talanta·2026
Same author

Cyclic lipopeptides from <i>Bacillus amyloliquefaciens</i> D-1 control leaf spot in <i>Pseudostellaria heterophylla</i> via enhancing host immunity and inhibiting <i>Alternaria alternata</i>.

Frontiers in microbiology·2026
Same author

Highly Sensitive Detection of Chymotrypsin Using Gold Nanoclusters with Peptide Sensors.

Micromachines·2026
Same author

<i>ATG5</i> gene regulates testosterone synthesis of testicular Leydig cells in Hezuo pig.

Frontiers in veterinary science·2025

Related Experiment Video

Updated: Jan 27, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K

Research progress of single molecule sensors based on DNA platform.

Yu Huang1, Muhammad Zeeshan Tahir1, Vesna Antic2

  • 1School of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.

Talanta
|January 25, 2026
PubMed
Summary

Deoxyribonucleic acid (DNA) is a programmable nanomaterial for single-molecule biosensing. Electrical and optical methods offer label-free and ultrasensitive detection, advancing molecular analysis platforms.

Keywords:
BiosensorDNAElectrochemical biosensorOptical sensorsSingle-molecule electrical measurementSingle-molecule sensors

More Related Videos

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.9K
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

23.1K

Related Experiment Videos

Last Updated: Jan 27, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.9K
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

23.1K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Deoxyribonucleic acid (DNA) is recognized as a fundamental molecule and a programmable nanomaterial.
  • DNA's self-assembly properties enable its use in advanced nanotechnology.
  • Single-molecule detection techniques integrated with DNA nanotechnology allow precise probing of molecular properties.

Purpose of the Study:

  • To review the working principles of electrical and optical detection strategies for DNA-based single-molecule biosensing.
  • To explore system construction approaches and evaluate application performance.
  • To summarize the evolution of DNA-based single-molecule biosensing platforms.

Main Methods:

  • Electrical sensing: Single-molecule junctions (SMJs), field-effect transistors (FETs), and nanopore technologies for label-free detection via electron transport, field-effect modulation, or ionic current.
  • Optical sensing: Surface-enhanced Raman scattering (SERS), fluorescence, and electrochemiluminescence (ECL) for ultrasensitive detection via optical signal changes.
  • DNA as recognition element: Leveraging DNA's specificity, programmability, and signal stability in optical platforms.

Main Results:

  • Electrical sensors enable label-free detection of single molecules.
  • Optical sensors provide ultrasensitive biomolecular recognition.
  • DNA-based recognition elements enhance specificity, programmability, and signal stability in biosensing platforms.

Conclusions:

  • Integrated multimodal analysis offers a robust framework for developing sensitive and selective single-molecule sensing platforms.
  • Despite challenges in stability and repeatability, DNA nanotechnology is pivotal in advancing biosensing.
  • Future directions involve overcoming limitations for reliable performance in complex environments.