Related Experiment Video
Updated: Jan 27, 2026
14:03
High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
Published on: March 24, 2023
2.4K
Evaluation of Pyrene Induced Cardiotoxicity Using Dual-Cardiotoxicity Evaluation Method
Hyewon Shin1, C-Yoon Kim1, Hyung Min Chung2
1College of Veterinary Medicine, Konkuk University, Seoul, Korea.
International Journal of Stem Cells
|January 26, 2026
Summary
Pyrene (Pyr), an environmental pollutant, causes functional heart problems even with short exposure. This study used human stem cell-derived cardiomyocytes to reveal Pyr
Area of Science:
- Environmental Toxicology
- Cardiovascular Research
- Stem Cell Biology
Background:
- Polycyclic aromatic hydrocarbons, like pyrene (Pyr), are environmental pollutants from incomplete combustion.
- Pyr exposure can harm various physiological systems, but its cardiotoxicity is not well understood.
- Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer a model for studying cardiac effects.
Purpose of the Study:
- To investigate the acute cardiotoxic effects of pyrene (Pyr) on human cardiomyocytes.
- To evaluate functional parameters and cell viability following Pyr exposure.
- To assess the utility of a microelectrode array for cardiotoxicity testing.
Main Methods:
- Exposure of hiPSC-CMs to varying doses of pyrene (Pyr).
- Assessment of cell viability and functional parameters.
- Simultaneous measurement of electrophysiological signals and contractility using a microelectrode array.
Main Results:
- Pyrene (Pyr) did not impact cell viability in short-term exposures.
- Pyr dose-dependently decreased beat period and field potential duration.
- Pyr progressively reduced spike amplitude and altered excitation-contraction coupling.
Conclusions:
- Pyrene (Pyr) induces functional cardiotoxicity upon acute exposure.
- The microelectrode array is valuable for assessing cardiotoxicity of environmental pollutants.
- Findings aid in developing safer alternative substances by understanding Pyr's cardiac impact.
Related Concept Videos
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
01:15Nursing Evaluation
4.2K
The evaluation stage signals the end of the nursing process. The nurse gathers evaluative data to assess whether or not the patient has attained the expected results. Whereas the nurse collects data in the nursing assessment to identify the patient's health concerns, the evaluation stage data determines if the indicated health issues are resolved. Evaluative data collection includes two sections: the data acquired to evaluate patient outcomes and the time criteria for data collection.
4.2K
01:29Self-Evaluation Maintenance Model
295
The Self-Evaluation Maintenance (SEM) model offers a psychological framework to understand how individuals’ self-esteem is influenced by the achievements of others, particularly those with whom they share close personal bonds. The SEM model operates when personal rather than social identity guides individuals. Central to this model is the notion that individuals have an inherent desire to preserve a favorable self-image, which is continuously shaped by interpersonal comparisons and...
295
01:30Dysrhythmias V: Evaluating Dysrhythmias
349
Dysrhythmias, also known as arrhythmias, are disturbances in the heart's rhythm that range from benign to life-threatening. A thorough evaluation is crucial for appropriate management and involves a comprehensive medical history, physical examination, and various diagnostic tests.Medical HistorySymptoms: Collect detailed information on palpitations, dizziness, syncope, chest pain, and fatigue. Note their onset, frequency, and triggers.Previous Cardiac Issues: Document any history of heart...
349
01:30Social Foundations of Self III: Self-Evaluation
188
Self-evaluation is the process by which individuals assess their abilities, behaviors, and characteristics based on feedback from others. Charles H. Cooley observed that a person’s self-perception is primarily influenced by how others see and judge them. He suggested that individuals form their identities based on their interpretations of others' reactions. As a result, social interactions play a crucial role in shaping self-esteem and personal identity. These external evaluations often...
188
01:30Heart Failure IV: Classification and Diagnostic Evaluation
359
Heart failure can be classified in various ways, with the most common classifications based on physical activity limitations, disease progression, severity, and treatment strategies.The Functional Classification of Heart Failure divides patients into four categories based on physical activity limitation due to symptom burden.Class I: Patients in this class have cardiac disease but no physical activity limitations. Ordinary activities like walking, climbing stairs, or routine tasks do not cause...
359