Related Experiment Video
Updated: Feb 4, 2026
10:30
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: August 4, 2022
3.5K
Integrated Electrophysiological and Optical Analysis of Human iPSC-Derived Cardiomyocytes Using Transparent ITO
Seul-Gi Lee1, Shinhye Park1, C-Yoon Kim1
1College of Veterinary Medicine, Konkuk University, Seoul, Korea.
International Journal of Stem Cells
|February 2, 2026
Summary
New microelectrode array chips using transparent indium tin oxide electrodes enable simultaneous monitoring of cell structure and electrical activity. This advances preclinical cardiotoxicity testing for safer drug development.
Area of Science:
- Biotechnology
- Cardiovascular Research
- Drug Development
Background:
- Preclinical cardiotoxicity assessment is vital in drug development, with a growing need for non-animal testing methods.
- Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) provide a relevant in vitro model for evaluating drug effects on the heart.
- Conventional microelectrode array (MEA) chips use opaque metal electrodes, hindering simultaneous cell morphology observation.
Purpose of the Study:
- To develop and validate a novel microelectrode array (MEA) platform for simultaneous electrophysiological and morphological assessment of cardiomyocytes.
- To investigate the utility of indium tin oxide (ITO) electrodes in MEA chips for preclinical cardiotoxicity evaluation.
- To compare the responses of iPSC-CMs to ion channel blockers and cardiotoxic drugs using the developed ITO-MEA system.
Main Methods:
- Fabrication of MEA chips with transparent and conductive indium tin oxide (ITO) electrodes.
- Culture and electrophysiological recording of human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) on ITO-MEA chips.
- Exposure of iPSC-CMs to ion channel blockers and known cardiotoxic drugs, followed by simultaneous monitoring of field potentials (FPs) and cell morphology.
Main Results:
- ITO-MEA chips enabled stable electrophysiological recordings and observation of iPSC-CM self-organization.
- Ion channel blockers induced dose-dependent changes in FPs without significant morphological alterations.
- Cardiotoxic drugs caused observable morphological damage, reduced cell viability, and progressive changes in FP parameters.
Conclusions:
- Indium tin oxide (ITO)-based MEA chips represent a next-generation platform for comprehensive cardiotoxicity evaluation.
- This technology allows for real-time, simultaneous monitoring of drug-induced electrophysiological and morphological changes in iPSC-CMs.
- The ITO-MEA system offers a promising alternative to traditional methods, enhancing preclinical safety assessment in drug development.
Keywords:
CardiotoxicityElectrophysiological signalInduced pluripotent stem cells derived cardiomyocytesOptical analysisTransparent indium tin oxide microelectrode chipMore Related Videos
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