Related Experiment Video
Updated: Jan 28, 2026
08:23
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
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Discovery of the High-Affinity Aptamer for Candidalysin Using a Dual-Mode Colorimetric-SERS Platform
Yige Sun1,2,3, Canlan Zheng1,2, Yuxuan Shi1,2
1Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
Biosensors
|January 27, 2026
Summary
Researchers developed a novel dual-mode biosensor to identify high-affinity aptamers targeting the toxic Candidalysin peptide from Candida albicans. This advancement aids in developing new treatments for candidiasis by enabling efficient and safe toxin removal.
Area of Science:
- Biotechnology
- Nanotechnology
- Microbiology
Background:
- Candida albicans infections pose health risks due to the peptide toxin Candidalysin.
- Current therapeutics do not specifically target Candidalysin, necessitating new treatment strategies.
- High-affinity aptamers are needed for efficient and safe removal of Candidalysin.
Purpose of the Study:
- To develop a dual-mode biosensor for screening high-affinity aptamers against Candidalysin.
- To leverage gold nanoparticles (AuNPs) for enhanced aptamer detection.
- To identify a specific aptamer with high affinity for Candidalysin.
Main Methods:
- Development of a dual-mode biosensor utilizing gold nanoparticles (AuNPs).
- Application of localized surface plasmon resonance (LSPR) for colorimetric detection.
- Utilizing surface-enhanced Raman scattering (SERS) for signal detection.
- Screening aptamers for high affinity to Candidalysin.
Main Results:
- The dual-mode biosensor demonstrated enhanced detection accuracy by reducing false negatives.
- Identified a specific aptamer exhibiting high affinity for Candidalysin.
- Successfully leveraged LSPR and SERS phenomena for Candidalysin detection.
Conclusions:
- The developed aptamer shows promise for targeting Candidalysin, advancing candidiasis treatment.
- The dual-mode biosensor is effective for screening high-affinity aptamers.
- This work paves the way for novel therapeutic strategies against Candida infections.
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