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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Targets for Drug Action: Overview01:26

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Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
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Nursing Implementation01:15

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Implementation is the execution of the nursing care plan developed during the planning phase.
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Antihistamines are a class of drugs widely used to alleviate the symptoms of allergies, such as sneezing, itching, and nasal congestion. They work by inhibiting the actions of histamine, which is released by immune cells in response to allergenic substances or tissue injuries.
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Pulmonary Tuberculosis I01:29

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Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
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Pulmonary Tuberculosis II01:28

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Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
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Related Experiment Video

Updated: Jan 29, 2026

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
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Targeted Next-Generation Sequencing in Drug-Resistant Tuberculosis: WHO Guidance and Practical Implementation

Sungwon Jung1,2

  • 1Department of Genome Medicine and Science, Gachon University College of Medicine, Incheon 21565, Republic of Korea.

Biomedicines
|January 28, 2026
PubMed
Summary
This summary is machine-generated.

Targeted next-generation sequencing (tNGS) offers rapid drug susceptibility testing for drug-resistant tuberculosis (DR-TB). This method aids in quicker treatment decisions by identifying resistance patterns, complementing existing diagnostic approaches.

Keywords:
drug resistancetargeted NGStuberculosis

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Area of Science:

  • Molecular Diagnostics
  • Microbiology
  • Genomics

Background:

  • Drug-resistant tuberculosis (DR-TB) requires rapid and accurate diagnostics.
  • Existing methods like phenotypic drug susceptibility testing (pDST) can be slow.
  • Targeted next-generation sequencing (tNGS) emerges as a bridge between rapid tests and pDST.

Purpose of the Study:

  • To review the principles, workflows, and interpretation of tNGS for DR-TB.
  • To assess tNGS's role in the diagnostic pathway according to WHO guidelines.
  • To identify operational, economic, and research considerations for tNGS implementation.

Main Methods:

  • Review of tNGS principles, panel design, and platform workflows (Illumina, Nanopore).
  • Analysis of catalogue-based interpretation using the WHO Mycobacterium tuberculosis mutation catalogue.
  • Evaluation of pooled accuracy, discordance sources, and bioinformatics/QA/EQA requirements.

Main Results:

  • tNGS provides rapid molecular drug susceptibility testing (DST) for a broad drug panel.
  • It aids in identifying resistance to fluoroquinolones (FQ) and newer agents like bedaquiline (BDQ).
  • Indeterminate results necessitate further testing (pDST/WGS) for confirmation.

Conclusions:

  • tNGS, within validated frameworks, can significantly reduce time to effective DR-TB therapy.
  • It offers tiered resistance signals for newer drugs, guiding treatment decisions.
  • Further research is needed for culture-free tNGS, heteroresistance detection, and standardized data sharing.