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Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS.

Gerardo N Guerrero-Flores1,2, Fabio J Pacheco1, Veronica L Martinez Marignac3

  • 1Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento (CIICSAC), Facultad de Ciencias de la Salud, Universidad Adventista del Plata, 25 de Mayo 99, Libertador San Martín 3103, Argentina.

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|January 28, 2026
PubMed
Summary
This summary is machine-generated.

Serum levels of N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) vary by race, sex, age, and BMI. This study developed a precise LC-MS/MS method to quantify these glycosylation components.

Keywords:
BMILC-MS/MSManNAcNeu5Acagedietethnicitysex

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Human Physiology

Background:

  • N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) are crucial for glycosylation and physiological functions.
  • The influence of demographic factors like age, BMI, race, and sex on serum ManNAc and Neu5Ac levels remains largely uncharacterized.

Purpose of the Study:

  • To develop and validate a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous quantification of ManNAc and Neu5Ac in human serum.
  • To investigate the associations between serum ManNAc and Neu5Ac levels and demographic factors (age, BMI, race, sex) in a human cohort.

Main Methods:

  • Development and validation of a simultaneous LC-MS/MS assay for serum ManNAc and Neu5Ac.
  • Analysis of 155 serum samples from the Adventist Health Study 2 (AHS-2) cohort.
  • Statistical analysis to determine correlations between analyte levels and demographic variables.

Main Results:

  • Serum Neu5Ac levels were significantly higher in women (p=0.029 for conjugated, p=0.026 for total).
  • Conjugated and total ManNAc levels were significantly higher in Black participants compared to White participants (p < 0.001).
  • Higher BMI was associated with increased free ManNAc (p=0.041), and older age was associated with higher free and total ManNAc (p ≤ 0.001 and p=0.045, respectively).

Conclusions:

  • The validated LC-MS/MS method provides sensitive and specific quantification of serum ManNAc and Neu5Ac.
  • Serum concentrations of ManNAc and Neu5Ac are influenced by race, sex, age, and BMI.
  • This methodology will enable further research into the physiological roles and clinical relevance of these glycosylation components.