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Updated: Jul 2, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 12, 2010
[Gibberellin oxidase gene PthGA2ox19 in hybrid 'Poplar 741' regulates plant growth and development]
Xiaoning Zhang1,2, Zhi'an Wang3, Ye Tang2
1College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Abstract:
Gibberellin 2-oxidase (GA2ox) is a key enzyme regulating the metabolism of gibberellic acid (GAs) in plants. Identifying GA2ox genes in poplar and analyzing their functions in regulating plant growth and development can provide technical support for breeding new poplar varieties. In this study, bioinformatics methods were used to identify and analyze GA2ox genes in hybrid 'Poplar 741'. A total of 34 GA2ox genes were identified, which were distributed on 7 pairs of chromosomes of hybrid 'Poplar 741'. The tissue expression pattern and GA3-induced expression pattern of PthGA2ox19 were analyzed by quantitative real-time PCR (qRT-PCR). It was found that PthGA2ox19 was highly expressed in stems and its expression level was significantly increased under GA3 induction. The overexpression vector of PthGA2ox19 was constructed and transformed into poplar by Agrobacterium transformation method. It was found that the expression level of PthGA2ox19 in transgenic lines was significantly higher than that in wild-type plants, and the phenotypes showed shorter plant height, thinner stem, shorter internodes and smaller leaves. The development of vascular tissues in transgenic plants was analyzed by paraffin section and microscope observation. The analysis of vascular tissue development showed that the vascular bundles of transgenic plants developed abnormally, the diameter of vessels decreased, and the thickness of xylem and phloem became thinner. In this study, 34 GA2ox genes of hybrid 'Poplar 741' were identified. The overexpression of PthGA2ox19 inhibited the growth and vascular tissue development of poplar, indicating that poplar GA2ox is involved in the regulation of plant growth and development, which can provide a new way for poplar plant type breeding.
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