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Updated: Jan 31, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
A multi-rotor near-infrared probe for high-sensitivity imaging of mitochondrial viscosity in epilepsy
Pengpeng Lu1, Yuening Wang1, Yan Huang2
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, P.R. China. lmzhang@chem.ecnu.edu.cn.
Abstract:
A mitochondria-targeted near-infrared fluorescent probe (Cy-TPE) is developed for mitochondrial viscosity imaging. A TICT-mediated multi-rotor architecture markedly enhances sensitivity to subtle viscosity changes, while persistent mitochondrial targeting enables long-term imaging. Cy-TPE visualizes elevated mitochondrial viscosity in epileptic neurons and mouse hippocampus in vivo.
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