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Updated: Feb 1, 2026

Electrostatic Method to Remove Particulate Organic Matter from Soil
Published on: February 10, 2021
Stereoselective behavior, residue dissipation, and household removal of isocycloseram stereoisomers in cabbage and
Wenwen Zhou1, Senyao Liu2, Jian Chen1
1College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Abstract:
An analytical approach was developed by coupling QuEChERS extraction with UHPLC-MS/MS to separate and quantify four stereoisomers of isocycloseram in cabbage and soil. The stereoisomers, including cis-(+)-(5R,4S) ISR, cis-(-)-(5S,4R) ISM, trans-(+)-(5R,4R) ISO, and trans-(-)-(5S,4S) ISS, were baseline-resolved on a Chiralcel OJ-3R column using acetonitrile/water (60:40, 0.3% formic acid). The method showed good linearity (R2 > 0.998), LOQs of 0.01 mg kg-1, and recoveries of 85.33-109.51% (RSDs ≤11.64%). Field trials revealed that cis-isomers, particularly ISM, dissipated faster than trans-isomers (ISO, ISS), with half-lives of 2.11-4.95 days. DFT calculations indicated a lower HOMO-LUMO gap and reduced stability for ISM, with ISS also showing lower stability. Washing with 1% citric acid removed up to 88.35% of ISM and over 76% of the other isomers, correlating with molecular polarity and electrostatic potential. This study provides a tool for chiral pesticide analysis and insights into stereoselective behavior, aiding environmental fate and dietary risk assessment.
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