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Updated: Feb 3, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
S-Nitrosation-Based Target Discovery and Drug Development: Toward a Nitric Oxide Drug 2.0 Paradigm
Hui Ye1, Chen Zhang1, Yihua Zhang1
1Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, P. R. China.
None:
Protein S-nitrosation (SNO) has emerged as a regulatory mechanism of nitric oxide (NO) signaling as important as the classical sGC/cGMP axis. Herein, we discuss recent advances that redefine SNO as an orchestrated, enzyme-modulated redox signal with therapeutic implications. We highlight the identification of the SCAN enzyme as a transnitrosylase, disease-associated SNO targets revealed through proteomic studies, and evolving pharmacological strategies─from global SNO modulation to protein- and site-specific interventions. Emerging platforms such as SNOTAC and warhead exemplify a "Nitric Oxide Drug 2.0" paradigm, enabling precise redox modulation for targeted drug discovery.
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