Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
Multi-input and Multi-variable systems01:22

Multi-input and Multi-variable systems

423
Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
In the absence of...
423
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.5K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.5K
Depth Perception and Spatial Vision01:15

Depth Perception and Spatial Vision

2.0K
Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.
2.0K
Parameters Affecting Nonlinear Elimination: Zero-Order Input, First-Order Absorption and Two-Compartment Model01:13

Parameters Affecting Nonlinear Elimination: Zero-Order Input, First-Order Absorption and Two-Compartment Model

318
Drugs administered through various routes can lead to nonlinear elimination, resulting in complex pharmacokinetic behaviors crucial to understanding efficacious drug dosing.
When a drug is administered through a constant intravenous infusion and eliminated via nonlinear pharmacokinetics, it follows zero-order input. For example, oral drugs undergo first-order absorption upon administration and are eliminated through nonlinear pharmacokinetics.
In the case of subcutaneously administered drugs,...
318
Tissues01:18

Tissues

85.3K
Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
85.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer.

Cell death discovery·2026
Same author

Deep visual proteomics uncovers nociceptor diversity and pain targets.

Nature communications·2026
Same author

Lipid nanoparticle-based non-viral in situ gene editing of congenital ichthyosis-causing mutations in human skin models.

Cell stem cell·2026
Same author

The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods.

eLife·2026
Same author

An Ultrasensitive Spatial Tissue Proteomics Workflow Exceeding 100 Proteomes Per Day.

Molecular & cellular proteomics : MCP·2025
Same author

Author Correction: Spatial proteomics of ovarian cancer precursors delineates early disease changes and drug targets.

Molecular systems biology·2025

Related Experiment Video

Updated: Feb 4, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

4.6K

Immunofluorescence Imaging-Guided Laser Microdissection for Ultralow Input Spatial Tissue Proteomics.

Sonja Fritzsche1,2, Fabian Coscia3

  • 1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2026
PubMed
Summary

This study presents a new protocol for laser microdissection (LMD) combined with immunofluorescence imaging and mass spectrometry. This method allows for precise proteomic analysis of specific cells from formalin-fixed paraffin-embedded (FFPE) tissues.

Keywords:
Deep visual proteomicsImmunofluorescence stainingLaser microdissectionTissue proteomics

More Related Videos

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.4K

Related Experiment Videos

Last Updated: Feb 4, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

4.6K
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.4K

Area of Science:

  • Biomedical research
  • Proteomics
  • Tissue analysis

Background:

  • Laser microdissection (LMD) is crucial for isolating specific cells from tissue sections for molecular analysis.
  • High spatial resolution is needed for accurate downstream analyses.
  • Formalin-fixed paraffin-embedded (FFPE) tissues are widely used but challenging for molecular studies.

Purpose of the Study:

  • To describe an optimized protocol for proteomic analysis of FFPE tissue sections.
  • To combine whole-slide immunofluorescence imaging with LMD and mass spectrometry.
  • To enable high-resolution molecular profiling of specific cellular compartments.

Main Methods:

  • Whole-slide immunofluorescence imaging was performed on FFPE tissue sections.
  • Laser microdissection (LMD) was used to isolate regions of interest.
  • Low-input liquid chromatography (LC) mass spectrometry (MS)-based proteomics was applied to the isolated samples.

Main Results:

  • The protocol successfully combined immunofluorescence imaging, LMD, and LC-MS proteomics.
  • Precise isolation of specific cells or regions was achieved.
  • High-resolution proteomic data was obtained from FFPE tissues.

Conclusions:

  • This optimized protocol facilitates in-depth proteomic analysis of FFPE tissues with high spatial resolution.
  • The method is valuable for studying cellular heterogeneity and biomarker discovery.
  • It advances molecular pathology and precision medicine research.