GFOGER-Modified PLGA/HA Electrospun Scaffolds Facilitate BMSCs' Osteogenic Differentiation

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA) biomaterials are established in tissue engineering.
• Functionalization with bioactive peptides like GFOGER can enhance osteogenic differentiation and angiogenesis in bone regeneration scaffolds.

Purpose of the Study:

• To fabricate and characterize a PLGA/HA electrospun scaffold functionalized with the GFOGER peptide.
• To evaluate the cytocompatibility and osteogenic potential of the PLGA/HA/GFOGER scaffold for bone regeneration.

Main Methods:

• Fabrication of PLGA/HA electrospun scaffolds.
• Surface functionalization with GFOGER peptide.
• Characterization using SEM, EDX, XPS, XRD, FTIR, TGA, and contact angle analysis.
• Cytocompatibility assessment via MTT assay.
• Evaluation of osteogenic differentiation markers (Runx2, BMP2, OCN) in bone marrow mesenchymal stromal cells (BMSCs).

Main Results:

• The PLGA/HA/GFOGER scaffolds were successfully fabricated and characterized for surface morphology, composition, and structural properties.
• The scaffolds demonstrated good cytocompatibility with BMSCs.
• BMSCs cultured on PLGA/HA/GFOGER scaffolds showed significantly enhanced osteogenic differentiation.

Conclusions:

• The PLGA/HA/GFOGER composite scaffold is a promising biomaterial for bone regeneration.
• GFOGER peptide functionalization effectively promotes osteogenic differentiation of BMSCs on PLGA/HA scaffolds.
• This approach holds potential for advancing bone tissue engineering applications.