Related Experiment Video
Updated: Feb 7, 2026

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
Published on: September 15, 2023
The Application of RenSeq for Pan-NLRome Analysis
Yujing Wu1,2, Jonathan D G Jones3, Xiao Lin4,5
1Department of Agri-microbiomics and Biotechnology, State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
None:
Resistance gene enrichment sequencing (RenSeq) was developed in 2013. It has accelerated the cloning of plant NLR genes and has contributed to resistance breeding for multiple crop plants, such as potato, wheat, and rice. By combining with other strategies, many applications were developed, such as cDNA-RenSeq, dRenSeq, SMRT-RenSeq, RLP/KSeq, AgRenSeq, and MutRenSeq. These methods have been widely applied in different crops. In this protocol, we present a step-by-step guide for applying RenSeq in gene cloning and Pan-NLRome analysis. The protocol covers bait design, library preparation, target enrichment, and downstream bioinformatic analysis. This methodology can make RenSeq more accessible to researchers working with different crops and enhance our understanding of plant resistance genes in the age of pan-genome.
Related Concept Videos
Photoluminescence: Applications
Radiation: Applications
The average...
Applications of Logarithms
Applications of Stress
The...
Applications of RC Circuits
Bernoulli's Principle: Applications
Entrainment devices use a high fluid speed to create low pressures and, thus, entrain one fluid into another. Some examples of these devices are given below:

