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Updated: Feb 7, 2026

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
Split-TurboID-Based Proximity Labeling for Probing the Composition of Spatiotemporally Defined Protein Complexes In
Zhiyan Wen1, Xinyu Zhang1, Yuexiao Li1
1State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, China.
Abstract:
Proteins frequently interact with other proteins to execute specific biological processes. Identifying protein-protein interactions (PPIs) is essential for understanding the molecular pathways underlying various physiological functions. Proximity labeling (PL), particularly the TurboID-based PL approach, has been widely utilized to identify PPIs across different organisms in recent years. For the first time, we applied the TurboID-based PL method to detect PPIs in plants. We recently demonstrated that the TurboID complementation approach, referred to as split-TurboID, can be used to screen for binding partners of defined protein complexes in planta. Here, we provide a detailed protocol for performing split-TurboID-based PL assays in Nicotiana benthamiana plants.
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