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Updated: Feb 11, 2026

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis
Published on: February 2, 2021
Morphological Alterations of Dopaminergic Neurons in the Substantia Nigra of Spontaneously Hypertensive Rats
P V Kulikova1, V V Guselnikova2, D E Korzhevskii1
1Institute of Experimental Medicine, St. Petersburg, Russia.
Abstract:
Morphological alterations of dopaminergic neurons in the substantia nigra were examined in spontaneously hypertensive rats (SHR) and normotensive Wistar rats. Dopaminergic neurons were identified by immunohistochemical staining for tyrosine hydroxylase. The study revealed a detrimental effect of arterial hypertension on the integrity of dopaminergic neurons in the substantia nigra. The number of neurons exhibiting signs of degeneration in the substantia nigra of SHR was significantly higher than that in control Wistar rats. These findings suggest a potential role of arterial hypertension in the pathogenesis of neurodegeneration in the substantia nigra.
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