Related Experiment Video
Updated: Feb 13, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei
Binbin Cui1, Xiayu Chen2, Mingfang Wang2
1Pharmacy Department, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo 315046, China; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
Abstract:
Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei. Technical assays include microscale thermophoresis (MST), quantitative reverse-transcription PCR (RT-qPCR), ultra-high-performance liquid chromatography-mass spectrometry (LC-MS), electrophoretic mobility shift assay (EMSA), bacterial adenylate cyclase two-hybrid (BACTH) assay, and molecular docking. For complete details on the use and execution of this protocol, please refer to Wang et al.1.
Related Concept Videos
Amplifying Signals via Second Messengers
What are Second Messengers?
Nucleotide Excision Repair
Cell-surface Signaling
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Bacterial Signaling

