Related Experiment Video
Updated: Feb 15, 2026
Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens
Published on: June 23, 2022
Artificial antibody-antigen-mediated lipase immobilization via a simplified affinity recognition strategy for
Hongli Chao1, Jing Wang2, Yi Wang2
1School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China.
Abstract:
Flavor esters are extensively used in food and cosmetic industries, yet their sustainable production remains challenging. This study developed a lipase bioreactor through artificial antibody-antigen-mediated immobilization for biocatalytic flavor ester synthesis via transesterification. Artificial antigens were prepared by lipase modification with p-nitrobenzaldehyde, while artificial antibodies were synthesized using 2-(4-nitrophenyl)-1,3-dioxolane (acetal-protected p-nitrobenzaldehyde analogue) as template molecules. These components self-assembled into immobilized lipase with 82.24 ± 0.13% immobilization efficiency, 17.59 ± 0.12 mg/g capacity, and 8.81 ± 0.27 U/mg specific activity. Integrating molecular simulations with acyl donor screening, the bioreactor achieved 97.18 ± 0.93% yield for cinnamyl acetate synthesis from cinnamyl alcohol and vinyl acetate. The system demonstrated exceptional continuous catalysis and scalability, confirming industrial translation potential. Successful synthesis of cinnamyl butyrate (86.25 ± 3.34%) and benzyl acetate (90.68 ± 2.25%) further established its versatility as a platform for diverse flavor ester production.
Related Concept Videos
01:41Affinity and Avidity
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:07Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
01:14Alkylation of β-Diester Enolates: Malonic Ester Synthesis
03:07Electron Affinity
01:15Dehydration Synthesis
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...