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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Real-time recombinase polymerase amplification (real-time RPA) detection of Staphylococcus aureus in foods
Qinglong Wang1, Liping Song1, Long Li2
1Beijing Food Inspection and Research Institute, Beijing Food Safety Monitoring and Risk Assessment Center, Beijing, China; Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation, Beijing, China.
Journal of Microbiological Methods
|February 14, 2026
Summary
A new real-time recombinase polymerase amplification (RPA) assay offers rapid, same-day detection of Staphylococcus aureus in food. This validated method provides reliable results comparable to traditional culture techniques.
Area of Science:
- Food microbiology
- Molecular diagnostics
- Bacteriology
Background:
- Staphylococcus aureus (S. aureus) is a significant foodborne pathogen.
- Accurate and rapid detection methods are crucial for food safety monitoring.
- Existing culture-based methods can be time-consuming.
Purpose of the Study:
- To develop and validate a rapid real-time recombinase polymerase amplification (RPA) assay for S. aureus detection.
- To evaluate the assay's performance in various food matrices.
- To compare the assay's efficacy against established reference methods.
Main Methods:
- Development of a real-time RPA assay targeting S. aureus.
- Validation according to GB4789.45 standards.
- Testing with artificially and naturally contaminated food samples (dairy, meat, cereals, soy, beverages).
- Comparison with GB 4789.10 culture method.
Main Results:
- The real-time RPA assay achieved specific amplification in 20 minutes at 39°C.
- The limit of detection (LOD₅₀) was 298 CFU/mL.
- Relative limits of detection (RLOD) were <2.5 across five food matrices after 6h enrichment.
- No significant difference was found between real-time RPA and the reference culture method (p > 0.05).
Conclusions:
- The developed real-time RPA assay provides rapid, same-day detection of S. aureus post-enrichment.
- The assay demonstrates diagnostic accuracy comparable to traditional culture methods.
- This real-time RPA assay is a reliable alternative for routine food safety monitoring of S. aureus.
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