Snapshot hyperspectral fundus imaging system using a microlens array

Retinal hyperspectral imaging holds significant promise for early disease diagnosis by quantifying the spectral signatures of metabolic and hemodynamic biomarkers. However, conventional hyperspectral imaging systems typically require extensive scanning, leading to prolonged acquisition times and rendering images susceptible to motion artifacts caused by involuntary eye movements. To address this limitation, we present a snapshot hyperspectral fundus camera employing a microlens array. The system features a streamlined optical architecture and compatibility with a standard commercial fundus camera across various field-of-view (FOV) configurations (20°, 35°, and 50°). Furthermore, the system allows a tunable balance between spectral resolution versus light throughput, enabling adaptation to a wide range of applications.