Effects of New Cellular Treatment Factor and Human Exosome Injection on Intact Skin Regeneration in a Randomized In...

Area of Science:

• Dermatology
• Aesthetics
• Regenerative Medicine

Background:

• Skin regeneration is a key focus in dermatology and aesthetics.
• Human exosomes and New Cellular Treatment Factor (NCTF) show regenerative potential.
• Combined effects of NCTF and exosomes on healthy skin are unstudied.

Purpose of the Study:

• Evaluate NCTF, two exosome products (CellExosome, ASCE+ Exosome), and their combinations on skin regeneration markers.
• Assess histological changes and microvascular density in intact skin.

Main Methods:

• Randomized, within-animal controlled study in 144 male Syrian golden hamsters.
• Intradermal injections of saline control, CellExosome, ASCE+ Exosome, NCTF, NCTF + CellExosome, or NCTF + ASCE+ Exosome.
• Skin biopsies analyzed via histology and CD34 immunohistochemistry at baseline and days 3, 7, 14.

Main Results:

• All active treatments significantly increased collagen synthesis and vascular proliferation by day 14 compared to control (P < 0.0001).
• NCTF + CellExosome combination yielded the highest improvements in collagen density and microvascularity.
• NCTF + ASCE+ Exosome and NCTF alone also showed significant regenerative effects.

Conclusions:

• NCTF and human-derived exosomes promote dermal regeneration and angiogenesis in intact skin.
• Combination therapy, especially NCTF + CellExosome, demonstrates synergistic effects for enhanced skin repair.
• Findings support further investigation for clinical aesthetic applications.