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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Updated: Feb 22, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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Structure-initiated CHA variant coordinating SDA for cascade amplification in CRISPR/Cas12a-based miRNA analysis.

Fei Yu1, Dan Yue1, Fanting Wang1

  • 1College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China.

Talanta
|February 20, 2026
PubMed
Summary

This study introduces a novel biosensing platform combining catalytic hairpin assembly (VCHA) and strand displacement amplification (SDA) for highly sensitive CRISPR/Cas12a-based microRNA detection. The platform achieves ultra-low detection limits for tumor biomarkers.

Keywords:
CRISPRCas12aMultiple signal amplificationStrand displacement amplification (SDA)Variant of catalytic hairpin assembly (VCHA)miRNA

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Area of Science:

  • Biomolecular Engineering
  • Molecular Diagnostics
  • Nucleic Acid Amplification

Background:

  • MicroRNAs (miRNAs) are crucial biomarkers for cancer diagnosis and monitoring.
  • Existing detection methods often face challenges in sensitivity and specificity.
  • Development of advanced biosensing platforms is essential for early disease detection.

Purpose of the Study:

  • To develop a novel, highly sensitive biosensing platform for microRNA detection.
  • To integrate variant catalytic hairpin assembly (VCHA) with strand displacement amplification (SDA) for enhanced signal amplification.
  • To utilize the CRISPR/Cas12a system for accurate and sensitive microRNA quantification.

Main Methods:

  • Engineering a VCHA system that initiates a cascade reaction upon target miRNA recognition.
  • Coordinating VCHA with SDA to generate abundant single-stranded activator DNA (acDNA).
  • Employing CRISPR/Cas12a trans-cleavage activity activated by acDNA for fluorescence readout.

Main Results:

  • The VCHA-SDA/Cas12a platform achieved an ultra-low limit of detection (0.166 pmol/L) for miRNA-155.
  • Demonstrated a broad dynamic range (1 pmol/L to 10 nmol/L) for miRNA detection.
  • Successfully quantified miRNA levels in clinical plasma samples and cell lines.

Conclusions:

  • The developed VCHA-SDA/Cas12a platform offers a robust and sensitive method for miRNA detection.
  • This platform shows significant potential for molecular diagnostics and clinical applications.
  • The synergistic cascade amplification strategy enhances detection capabilities for biomarkers.