Bio-inks with PRF Increase Human Osteosarcoma Cell Line (SaOS-2) Viability in Extrusion-Based 3D-Bioprinted...

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• 3D bioprinting is crucial for creating functional tissues and organs.
• Mechanical stress during extrusion bioprinting reduces cell viability.
• Enhancing cell survival in bioprinted constructs is critical for clinical applications.

Purpose of the Study:

• To evaluate osteoblast-like cell (SaOS-2) viability in alginate bioinks with different platelet concentrates.
• To identify bioink formulations that improve cell survival after 3D extrusion bioprinting.
• To assess the potential of platelet concentrates in bone tissue engineering bioinks.

Main Methods:

• Alginate bioinks were formulated with varying concentrations (10%, 20%) of platelet-rich plasma (PRP), platelet-poor plasma (PPP), platelet-rich fibrin (PRF), and injectable PRF (iPRF).
• Bioinks were rheologically characterized for printability.
• SaOS-2 cells were embedded and printed using extrusion-based 3D bioprinting.
• Cell viability was assessed using LIVE/DEAD assay and confocal microscopy at 24, 48, and 72 hours.

Main Results:

• Rheological analysis confirmed printability for 10% PPP, 10% PRF, and 20% PRF bioinks.
• Cell viability remained above 58% at 24 hours and 80% at 48 hours across all tested bioinks.
• PRF-containing constructs showed significant viability recovery up to 86% at 72 hours, indicating a protective effect.

Conclusions:

• PRF-enriched bioinks significantly enhance cell viability post-extrusion bioprinting.
• PRF improves the physical integrity of 3D bioprinted scaffolds.
• PRF-based bioinks show promise for clinical bone tissue engineering applications.