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Optimization of Bioactive Compound Extraction from Prunus spinosa L. Fruits Using Ultrasound-Assisted Extraction with
Asmaa Berkati1, Nadir Ben Hamiche1, Amina Kribeche1,2
1Université de Bejaia, Faculté des Sciences de la Nature Et de la Vie, Laboratoire de Biomathématique, Biochimie, Biophysique et Scientométrie (L3BS), Bejaia 06000, Algeria.
This study optimized glycerin-based ultrasound-assisted extraction (UAE) for bioactive compounds from Prunus spinosa fruits. Optimized conditions significantly enhanced total phenolic and flavonoid content and antioxidant activity, showcasing a sustainable extraction method.
Area of Science:
- Green chemistry and sustainable extraction technologies.
- Food science and nutraceutical development.
- Phytochemistry and natural product analysis.
Background:
- Wild fruits like Prunus spinosa are rich sources of bioactive compounds.
- Valorization of underutilized wild fruits aligns with green chemistry principles.
- Efficient and sustainable extraction methods are needed to harness fruit bioactives.
Purpose of the Study:
- To optimize glycerin-based ultrasound-assisted extraction (UAE) for bioactive compounds from Prunus spinosa L. fruits.
- To compare Response Surface Methodology (RSM) and Artificial Neural Networks (ANN) for process modeling.
- To validate optimal extraction conditions and characterize phytochemicals.
Main Methods:
- Glycerin-based ultrasound-assisted extraction (UAE) was employed.
- Response Surface Methodology (RSM) and Artificial Neural Networks (ANN) were used for optimization.
- UHPLC-DAD-HRMS/MS was utilized for phytochemical profiling.
Main Results:
- Optimized UAE significantly increased total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity.
- Artificial Neural Networks (ANN) showed slightly higher predictive accuracy than RSM.
- Optimal conditions identified: 9 min, 100% ultrasonic amplitude, 40% water in glycerin.
- Phytochemical analysis revealed dominance of phenolic acids, caffeoylquinic acid derivatives, and flavonol glycosides.
Conclusions:
- Glycerin-based UAE is an effective and sustainable method for extracting bioactive compounds from Prunus spinosa.
- The optimized process offers a food-safe alternative to traditional solvent extractions.
- Results support the use of Prunus spinosa extracts in functional foods, nutraceuticals, and cosmetics.
