Related Experiment Video
Updated: Feb 28, 2026

Scanning Electron Microscopic Evaluation of Surface Defects of Remover Retreatment File After Single and Multiple Uses
Published on: October 11, 2024
Ex vivo comparative analysis of retrievability among four calcium silicate-based sealers for regaining apical patency
Darian Shomali1, Timothy Kirkpatrick2, Sang Won Kwak3
1Department of Endodontics, School of Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Objectives:
: Efficient retrievability is a key requirement for endodontic sealers. This study evaluated the retrievability of four different calcium silicate-based sealers (CSS).
Methods:
A total of 153 single-rooted human teeth with straight canals were decoronated to a standardized working length of 12 mm. The canals were negotiated to working length using K files up to size 15/.02, followed by rotary instrumentation up to 35/.04, 2 mm short of working length. The teeth were randomly assigned to five groups: NeoSEALER Flo (NEO; Avalon Biomed), Ceraseal (CS; Meta Biomed), Endosequence BC Sealer (BC; Brasseler USA), AH Plus Bioceramic Sealer (AHB; Dentsply Sirona), and a negative control group. Sealer application and obturation with a 35/.04 gutta-percha cone were performed. After incubation at 37°C in 100% humidity for 7 days, retreatment was performed until apical patency was obtained, with retrievability assessed by regaining apical patency. One-way analysis of variance and Tukey contrast test were used to determine whether there was a significant difference among the four different CSS (p < 0.05).
Results:
Success rates in regaining apical patency were NEO (79.4%), CS (37.0%), BC (50.0%), and AHB (69.7%). NEO demonstrated the highest retrievability, while CS had the lowest (p < 0.01).
Conclusions:
The type of CSS used has a considerable impact on retreatment difficulty. Among the tested sealers, Neo- SEALER Flo showed the highest retrievability, making it the most retrievable CSS in terms of retreatment efficacy.

