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Related Experiment Video

Updated: Mar 4, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
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Selective Lipoprotein Removal Enables High-Purity EV Isolation from Plasma via Aptamer-Based Mesh Filtration.

SoYoung Jeon1, YongWoo Kim2, Sehyun Shin1,2,3

  • 1Department of Micro-Nanosystem Technology, Korea University, Seoul, Republic of Korea.

Small (Weinheim an Der Bergstrasse, Germany)
|March 3, 2026
PubMed
Summary

ApoFilter efficiently removes lipoproteins like HDL and LDL from extracellular vesicle (EV) samples using aptamer-based filtration. This method yields ultrapure EVs for advanced research and clinical applications.

Keywords:
affinityaptamerhigh‐purity EVslipoprotein removalmesh‐filtrationpurification

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Lipoproteins (e.g., HDL, LDL) are abundant in plasma and physically resemble extracellular vesicles (EVs).
  • This similarity poses a significant challenge for isolating pure EVs, hindering downstream applications.
  • Existing EV isolation methods often struggle with efficient lipoprotein depletion.

Purpose of the Study:

  • To develop a rapid and selective method for depleting lipoproteins during EV isolation.
  • To enhance the purity of extracellular vesicles (EVs) for research and translational use.
  • To address the bottleneck of lipoprotein contamination in EV purification workflows.

Main Methods:

  • ApoFilter, an aptamer-based mesh-filtration platform, was developed.
  • High-affinity aptamers targeting ApoA1 and ApoB100 were immobilized on nylon mesh.
  • The system operates in a spin-column format for rapid, gravity-driven separation.

Main Results:

  • ApoFilter achieved >99% removal of targeted lipoproteins (HDL, LDL) from plasma and purified samples.
  • The filtration process was rapid, taking only 1 minute.
  • EV integrity was maintained, with negligible capture of non-target components.
  • ApoFilter effectively reduced lipoprotein contaminants when integrated with ultracentrifugation, SEC, and ExoTFF.

Conclusions:

  • ApoFilter provides a simple, rapid, and effective solution for lipoprotein depletion in EV isolation.
  • The platform significantly improves EV purity, making them suitable for molecular analyses.
  • This technology addresses a critical challenge in EV research and facilitates translational applications.