Rumen microbiota-associated stress alleviation by creatine pyruvate in newly received cattle: a multi-omics study

Area of Science:

• Animal Science
• Microbiology
• Nutritional Biochemistry

Background:

• Transport stress in cattle weakens immune response and growth.
• Rumen microbiota imbalance exacerbates stress.
• Investigating creatine pyruvate (CrPyr) as a mitigation strategy is crucial for the beef industry.

Purpose of the Study:

• To elucidate the mechanisms by which CrPyr alleviates stress in newly received cattle.
• To assess the impact of CrPyr on rumen microbiota and host physiology.
• To validate CrPyr's efficacy through multi-omics and experimental approaches.

Main Methods:

• Multi-omics approaches: metagenomics and metabolomics.
• In vitro and in vivo experiments.
• Rumen microbiota transplantation (RMT) in mice.

Main Results:

• CrPyr significantly reduced stress hormones and inflammatory markers.
• Enhanced antioxidant capacity was observed with CrPyr supplementation.
• CrPyr increased Prevotella abundance, volatile fatty acid (VFA) production, and ATP synthesis.

Conclusions:

• CrPyr modulates ruminal Prevotella to enhance glutathione and VFA metabolism.
• CrPyr accelerates ATP and nucleotide synthesis, alleviating stress in cattle.
• CrPyr is an effective nutritional intervention for improving rumen function and livestock productivity.