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PABPC1 regulates EOPE pathogenesis through modulating TMBIM4 mRNA expression and stability.

Shuyu Zhao1, Nan Lv1, Tianyi Liu1

  • 1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150001, China.

European Journal of Medical Research
|March 5, 2026
PubMed
Summary
This summary is machine-generated.

Poly(A) Binding Protein Cytoplasmic 1 (PABPC1) suppresses early-onset preeclampsia (EOPE) by enhancing trophoblast cell proliferation and invasion. PABPC1 increases the stability of Transmembrane BAX Inhibitor Motif Containing 4 (TMBIM4) mRNA, offering a potential therapeutic strategy for EOPE.

Keywords:
Cell proliferation and invasionEOPEMRNA stabilityPABPC1TMBIM4

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Area of Science:

  • Obstetrics and Gynecology
  • Molecular Biology
  • Cell Biology

Background:

  • Preeclampsia (PE) is a severe pregnancy complication linked to inadequate trophoblast cell function.
  • Transmembrane BAX Inhibitor Motif Containing 4 (TMBIM4) is downregulated in early-onset preeclampsia (EOPE) placental tissues.
  • Poly(A) Binding Protein Cytoplasmic 1 (PABPC1) regulates mRNA stability and cell behavior.

Purpose of the Study:

  • To investigate the role of PABPC1 in trophoblast cell proliferation and invasion.
  • To determine how PABPC1 affects TMBIM4 mRNA stability.
  • To explore PABPC1 as a potential therapeutic target for EOPE.

Main Methods:

  • In vitro studies using HTR-8/SVneo cells with PABPC1 manipulation.
  • In vivo studies using a rat model of preeclampsia.
  • RIP-qPCR and dual luciferase reporter assays to confirm PABPC1-TMBIM4 interaction.

Main Results:

  • PABPC1 overexpression promoted trophoblast cell proliferation, migration, and invasion while inhibiting apoptosis.
  • PABPC1 upregulation ameliorated hypertension and kidney damage in a rat model.
  • PABPC1 enhances TMBIM4 mRNA stability by binding to its 3'UTR, and TMBIM4 is crucial for PABPC1's effects.

Conclusions:

  • PABPC1 suppresses EOPE by enhancing trophoblast proliferation and invasion via increased TMBIM4 mRNA stability.
  • PABPC1 plays a critical role in EOPE pathogenesis.
  • PABPC1 upregulation presents a promising therapeutic strategy for EOPE.