Light-Addressable Organic Photoelectrochemical Transistor: Toward Multiplex Screening of Inhibitor Drugs.

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Light-addressable detection is gaining traction, but its application in organic photoelectrochemical transistors (OPECTs) remains unexplored.
• OPECTs offer a promising platform for sensitive bioassays.

Purpose of the Study:

• To develop and demonstrate the first light-addressable OPECT for bioanalytical applications.
• To utilize this platform for benchmarking thrombin activity and screening inhibitor drugs.

Main Methods:

• Fabrication of a nine-channel light-addressable OPECT device incorporating a photoactive metal-organic framework heterojunction.
• Integration of alkaline phosphatase (ALP)-labeled peptides for thrombin detection.
• Monitoring ALP-enabled biocatalytic precipitation to modulate interfacial charge transfer and gating effects.

Main Results:

• The developed OPECT successfully detected thrombin activity.
• The device effectively differentiated the inhibition efficiencies of six distinct thrombin inhibitor drugs.
• Demonstrated parallel screening capabilities for drug discovery.

Conclusions:

• The first light-addressable OPECT has been successfully developed and validated for bioanalysis.
• This technology provides a novel platform for high-throughput screening of enzyme activity and drug inhibitors.
• Light-addressable OPECTs hold significant potential for future bioanalytical and diagnostic applications.