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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Molecular differentiation between Plinia cauliflora (Mart.) Kausel and Plinia trunciflora (O. Berg) Kausel using
Ana Kelly S Silva1, Yohan Fritsche1, Dalvan Carlos Beise1
1Universidade Federal de Santa Catarina, Departamento de Fitotecnia, Centro de Ciências Agrárias, Rod. Admar Gonzaga, 1346, 88034-000 Florianópolis, SC, Brazil.
Abstract:
Molecular markers are important tools for genetic studies, including evolution, species differentiation, and population genetics. The genus Plinia (Myrtaceae) consists of various fruit tree species, some of which are referred to as jaboticaba. The subtle interspecific morphological differences among some jaboticaba species make their taxonomy challenging. This study aimed to develop nuclear SSR markers to distinguish and analyze the genetics of two jaboticaba species, Plinia cauliflora and Plinia trunciflora. The developed markers - eight for P. cauliflora and seven for P. trunciflora - were cross-amplified in both species. Jointly, these markers presented high discriminatory power, private alleles at the species level, and distinguished populations of both species.
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