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Draft genome sequence and annotations of the mastitis-associated multidrug-resistant Staphylococcus aureus strain

Kh Yeashir Arafat1, Md Morshedur Rahman1, Sayeda Fowzia Homa1

  • 1Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.

Microbiology Resource Announcements
|March 12, 2026
PubMed

Insights

We sequenced the genome of multidrug-resistant Staphylococcus aureus from cow's milk. This bacterium possesses numerous antimicrobial resistance and virulence genes, indicating significant disease-causing potential.

Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Science

Background:

  • Bovine clinical mastitis (CM) is a significant concern in dairy farming.
  • Multidrug-resistant (MDR) Staphylococcus aureus strains pose a threat to animal and human health.
  • Understanding the genetic basis of MDR S. aureus is crucial for effective treatment and control.

Purpose of the Study:

  • To present the draft genome sequence of a multidrug-resistant Staphylococcus aureus strain (MBBL_MP1) isolated from bovine clinical mastitis.
  • To identify antimicrobial resistance genes (ARGs) and virulence factor genes (VFGs) within the genome.
  • To assess the pathogenic potential of the MBBL_MP1 strain.

Main Methods:

  • Whole-genome sequencing of Staphylococcus aureus MBBL_MP1.
  • Bioinformatic analysis to identify ARGs and VFGs.
  • Genome assembly and characterization.

Main Results:

  • The draft genome of S. aureus MBBL_MP1 is 2.75 Mb with a GC content of 32.5% and 153.4× coverage.
  • The genome harbors 29 antimicrobial resistance genes (ARGs).
  • The genome contains 65 virulence factor genes (VFGs), suggesting high pathogenic potential.

Conclusions:

  • The genome sequence provides valuable insights into the genetic makeup of MDR S. aureus from bovine CM.
  • The identified ARGs and VFGs highlight the strain's potential to cause severe infections and resist antibiotic treatment.
  • This genomic data can aid in developing targeted interventions for bovine mastitis and preventing the spread of antimicrobial resistance.
Keywords:
Staphylococcus aureusclinical mastitisdairy cowmilkmultidrug resistancevirulence

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