Related Experiment Video
Updated: Mar 14, 2026

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
Published on: September 19, 2013
Exploring the Modulation of Extracellular Metabolites in Different Listeria monocytogenes Strains under Cold-Stress
Hyun-Jun Kim1, Hye-Jin Kim1, Cheorun Jo1,2
1Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
None:
This study investigated the modulation of extracellular metabolites in Listeria monocytogenes NCCP 15743 (L5), NCCP 16594 (L6), and ATCC 19111 (L9) strains in cold-stressed culture. The strains were cultured in Mueller Hinton broth at 8°C for 22 d. Extracellular metabolites were extracted at five growth phases (initial, lag, log, early saturate, and saturate) of each strain. Under cold-stress, growth phases of L5 and L6 exhibited similarities, while L9 displayed a distinct pattern. The change in extracellular metabolites under cold-stress was dependent on growth phase and strain. The presence of L. monocytogenes was distinguished based on the concentrations of trehalose, isoleucine, arginine, and phenylalanine. During extended cold-stressed culture, all strains enhanced two metabolic pathways at the lag and log phases: energy metabolism (trehalose, lactate, propanoate, acetate, ethanol, and formic acid) and glutathione-related metabolism (acetate, histidine, arginine, proline, glutamate, glycine, serine, and methionine). The expression of these extracellular metabolites provides crucial insights into the complex metabolic adaptations of L. monocytogenes during cold-stress culture. This study introduces a distinctive approach to identifying L. monocytogenes under cold-stress, offering potential application for safety enhancement in the food industry.
Related Concept Videos
Stringent Response in E. coli
Other Stress Responses in Bacteria
Responses to Heat and Cold Stress
Factors Influencing Microbial Growth: Temperature
Physical Methods for Controlling Microbial Growth: Temperature
Gene Regulation During Sporulation

