Related Experiment Video
Updated: Mar 15, 2026

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
A ratiometric fluorescence sensor based on starch/polyacrylamide/deep eutectic solvent hydrogel for visual and
Xueting Li1, Yajie Bo2, Qichao Ye2
1State Key Laboratory of Green Papermaking and Resource Recycling, Shandong Key Laboratory of Healthy Food Resources Exploration and Creation, College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China; College of Biological and Chemical Engineering, Qilu Institute of Technology, Jinan 250200, China.
Abstract:
The abuse of organophosphorus pesticides, such as methyl parathion (MP), often leads to environmental pollution and food safety issues. It is necessary to establish a rapid and non-destructive method for MP detection. Herein, a starch-based ratiometric fluorescence hydrogel was constructed by introducing carbon quantum dots and Eu3+ as the detection and background fluorescence source, respectively. The starch-based hydrogel exhibited good mechanical performances with an elongation of 976% and a compression ratio of 70%, being validated by microscopic characterizations and density functional theory (DFT) calculations. Moreover, the high adsorption energy (-65.3 kcal/mol) obtained from DFT between the hydrogel and MP proved its high selectivity for MP. The visual sensor had a limit of detection of 0.4 μM, good sensitivity, stability and reusability for MP detection. This study provides a visual and nondestructive detection approach for determination of pesticide residues and guides intelligent application of fluorescent hydrogel-sensors.

