Protocol for targeted peripheral adeno-associated viral vector transduction of the intradental neurons in mice

Here, we present the methodology for surgical preparation of the mouse molar teeth and subsequent adeno-associated virus (AAV) transduction of intradental neurons within the tooth (AAV-i.d.). We describe steps for mouse positioning, microscope setup, drilling to expose pulp horns, and applying the AAV-silk mixture. We then detail procedures for composite placement to cover the preparation and trigeminal ganglion (TG) screening to visualize the surface and assess and verify AAV labeling. AAV-i.d. labels intradental neurons and traces their associated neuronal projections. For complete details on the use and execution of this protocol, please refer to Ronan et al.¹.