Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

9.4K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
9.4K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

19.3K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
19.3K
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

4.2K
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
4.2K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.8K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.8K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.3K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.3K
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

5.5K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated clinical and postmortem profiling in schizophrenia reveals a cognitive subtype linked to cerebrovascular disease.

Translational psychiatry·2026
Same author

Reducing sedentary behavior through workplace counseling: effects on activity, sitting breaks, and well-being - a randomized controlled trial.

Archives of public health = Archives belges de sante publique·2026
Same author

Divergent hippocampal output via covariant local and long-range neuronal structure.

Progress in neurobiology·2026
Same author

Omics-based approaches for acute brain injury research.

Current opinion in critical care·2026
Same author

Distinct Layer 6b transcriptomic subtypes parcellate the cortical mantle.

Progress in neurobiology·2025
Same author

Quantification and analysis of multiplexed fluorescence insitu hybridization data using open-source tools.

STAR protocols·2025
Same journal

A large brain adds new types of neurons: Molecular and functional signatures of spindle neurons in the human neocortex.

Trends in neurosciences·2026
Same journal

Exercise as a regulator of glymphatic function.

Trends in neurosciences·2026
Same journal

The neural basis of laughter.

Trends in neurosciences·2026
Same journal

Enteric neuroimmune interactions in health and disease.

Trends in neurosciences·2026
Same journal

Atomic insights into the physiological and functional diversity of NMDA receptors.

Trends in neurosciences·2026
Same journal

Cognitive functions of the GPe.

Trends in neurosciences·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons
09:27

Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons

Published on: October 10, 2025

646

The subiculum: cell-type-specific composition, computation, and function.

Adrienne I Kinman1, Larissa Kraus1, Mark S Cembrowski2

  • 1Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.

Trends in Neurosciences
|March 15, 2026
PubMed
Summary
This summary is machine-generated.

Researchers identified distinct excitatory neuron subtypes in the subiculum, a key hippocampal output region. These subtypes, organized spatially, offer new insights into learning, memory, and cognition mechanisms.

Keywords:
behaviorcircuitshippocampusmultimodalprojectiontranscriptome

More Related Videos

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
12:14

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Published on: September 14, 2022

8.6K
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

32.2K

Related Experiment Videos

Last Updated: Mar 17, 2026

Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons
09:27

Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons

Published on: October 10, 2025

646
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
12:14

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Published on: September 14, 2022

8.6K
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

32.2K

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Systems Neuroscience

Background:

  • The hippocampus is crucial for learning, memory, and cognition.
  • The subiculum is the main output region of the hippocampus, utilizing excitatory neurons.
  • Understanding subiculum excitatory neuron properties is key to deciphering hippocampal function.

Purpose of the Study:

  • To review and integrate recent rodent research on subiculum excitatory neurons.
  • To explore molecular, cellular, circuit, and functional properties of these neurons.
  • To establish a framework for understanding hippocampal processing and outputs.

Main Methods:

  • Literature review focusing on recent rodent studies.
  • Integration of findings across molecular, cellular, and circuit levels.
  • Analysis of functional properties of subiculum excitatory neurons.

Main Results:

  • Discrete excitatory neuron subtypes are coherently represented across modalities within the subiculum.
  • These identified subtypes occupy distinct spatial subdomains.
  • Evidence suggests spatially restricted parallel outputs from the hippocampus.

Conclusions:

  • A subtype framework for subiculum excitatory neurons helps contextualize hippocampal processing.
  • This framework supports the concept of spatially segregated hippocampal outputs.
  • Provides a roadmap for future research into hippocampal mechanisms of function.