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SlNOR interacts with SlMYB60 to regulate the jasmonic acid-mediated low-temperature stress response in tomato
Xiangguang Meng1,2,3, Zhen Kang1,2,3, Guo Chen1,2,3
1College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Plant Physiology
|March 16, 2026
Summary
Tomato plants enhance low-temperature tolerance through a jasmonic acid (JA) biosynthesis pathway. The SlMYC2-SlNOR-SlMYB60-SlLOX10/11 module regulates JA accumulation, crucial for plant stress response.
Area of Science:
- Plant Biology
- Molecular Biology
- Stress Physiology
Background:
- Low-temperature stress severely impacts plant growth and development.
- NAC transcription factors (TFs) are vital for plant stress tolerance, including responses to low-temperature, drought, and salinity.
- These TFs initiate stress responses and signal transduction pathways.
Purpose of the Study:
- To investigate the role of the NAC transcription factor gene NON RIPENING (SlNOR) in tomato's low-temperature tolerance.
- To elucidate the molecular mechanism by which SlNOR regulates jasmonic acid (JA) biosynthesis and low-temperature response.
- To identify key regulatory components and interactions within the JA-mediated stress response pathway.
Main Methods:
- Gene expression analysis to determine SlNOR induction by low-temperature and JA.
- Biochemical assays to confirm SlNOR's direct targeting of LIPOXYGENASE 10/11 (SlLOX10/SlLOX11).
- Protein-protein interaction studies (e.g., yeast two-hybrid) to assess SlNOR and SlMYB60 interaction.
- Analysis of MYELOCYTOMATOSIS 2 (SlMYC2) binding to SlNOR and its effect on SlNOR expression.
Main Results:
- SlNOR expression is significantly induced by low-temperature and JA.
- SlNOR promotes JA biosynthesis by regulating SlLOX10/SlLOX11, enhancing low-temperature tolerance.
- Overexpression of SlNOR and SlLOX11 increased JA accumulation and reduced reactive oxygen species (ROS) under low-temperature stress.
- SlNOR interacts with SlMYB60, which also targets SlLOX10/SlLOX11, with similar effects on phenotype.
- SlMYC2 directly binds to SlNOR, promoting its expression, highlighting a regulatory module.
Conclusions:
- The SlMYC2-SlNOR-SlMYB60-SlLOX10/11 module is central to regulating JA synthesis in tomato.
- This module plays a critical role in mediating the low-temperature stress response by controlling JA accumulation.
- Understanding this pathway provides insights into improving plant tolerance to abiotic stresses.
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