Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

963
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
963
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compact neural network algorithm for electrocardiogram classification.

Physical and engineering sciences in medicine·2026
Same author

Nanophotonic Glyphosate Sensing Integrated into a Portable Optoelectronic Device with Deep-Learning Imaging.

ACS sensors·2026
Same author

Sample-Half-Inserted Quantum Interferometer.

Physical review letters·2026
Same author

Smart Machine Vision for Universal Spatial-Mode Reconstruction.

IEEE transactions on neural networks and learning systems·2025
Same author

Conditional quantum plasmonic sensing.

Nanophotonics (Berlin, Germany)·2024
Same author

Compressive characterization of telecom photon pairs in the spatial and spectral degrees of freedom.

Optica·2024
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

Related Experiment Video

Updated: Mar 19, 2026

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

8.1K

High-density three-dimensional image reconstruction using rapid modulation of light.

Jorge-Alberto Peralta-Ángeles, Mingyuan Hong, Mario A Quiroz-Juárez

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |March 17, 2026
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method for high-density 3D holographic reconstruction using digital micromirror devices (DMDs). It effectively eliminates layer crosstalk without extra optics, enabling compact, high-resolution 3D holographic projectors.

    More Related Videos

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.7K
    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    16.1K

    Related Experiment Videos

    Last Updated: Mar 19, 2026

    Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
    07:27

    Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

    Published on: November 21, 2016

    8.1K
    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.7K
    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    16.1K

    Area of Science:

    • Optics and Photonics
    • 3D Imaging Technologies

    Background:

    • Digital holography reconstructs 3D images using spatial light modulators but suffers from layer crosstalk due to coherent light sources.
    • Existing methods to reduce crosstalk often require additional optical components, increasing system complexity.

    Purpose of the Study:

    • To present a novel method for high-density 3D object reconstruction using rapid light field modulation.
    • To eliminate coherence-induced multiplane crosstalk in holographic displays without additional optical elements.

    Main Methods:

    • Utilized digital micromirror devices (DMDs) for rapid modulation of light fields at approximately 10 kHz.
    • Discretized 3D objects into multiplane light-point contours, controlling resolution via light point density.
    • Employed independent amplitude holograms for spatial and temporal control of each 3D image point.

    Main Results:

    • Successfully eliminated multiplane crosstalk inherent in coherent holographic reconstruction.
    • Achieved high-density 3D reconstruction without the need for supplementary optical resources.
    • Demonstrated a versatile and simple method for 3D holographic projection.

    Conclusions:

    • The proposed DMD-based method offers a practical solution for high-resolution 3D holographic displays.
    • This technique overcomes limitations of traditional holography, paving the way for compact holographic projectors.
    • The elimination of crosstalk enhances image quality and full-depth control in 3D reconstructions.