Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Larynx01:21

Larynx

The human larynx, often referred to as the voice box, is an intricate organ located in the neck. It serves as a pathway for air to enter the lungs during respiration and is an essential component of voice production.
Anatomy of the Larynx
The larynx consists of various components, including cartilage, muscles, and vocal cords. Its structure includes three large unpaired cartilages—the thyroid, cricoid, and epiglottis—and three smaller paired cartilages—the arytenoids, corniculates, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Citrus sunki Peel Extract Enhances Proliferation and Differentiation of Fibro-Adipocyte Progenitors in Holstein Cattle for Cultivated Meat Production.

Food science of animal resources·2026
Same author

Ambient fine particulate matter (PM2.5) induces AhR-dependent proinflammatory responses and reactive oxygen species production in human conjunctival epithelial cells.

Cutaneous and ocular toxicology·2026
Same author

Surgery-Based Salvage for Locoregional Recurrent Salivary Gland Carcinoma: Comparative Survival Evidence from a Systematic Review and Meta-analysis.

Clinical and experimental otorhinolaryngology·2026
Same author

Human Turbinate Mesenchymal Stromal Cell-Derived Exosomes Alleviate PM2.5-Induced Pyroptosis via Promoting Mitophagy in Human Vocal Fold Fibroblasts.

Journal of voice : official journal of the Voice Foundation·2026
Same author

Polystyrene Nanoplastics Induce Inflammasome Activation in Nasal Epithelial Cells via ROS-Mediated Mitochondrial Dysfunction.

Environmental toxicology·2026
Same author

Precision-enhancing magnetic needle tracking system in ultrasound-guided fine needle aspiration of head and neck lesions: a novel image-guided technique.

Endocrine·2026
Same journal

Physiological Validation of Glottal Airflow Estimation Using Neck-Surface Accelerometry: A Comparison With Electroglottographic Measures.

Journal of voice : official journal of the Voice Foundation·2026
Same journal

Voice Activity and Participation Profile in Teachers with and without Risk of Voice Disorders in Türkiye.

Journal of voice : official journal of the Voice Foundation·2026
Same journal

Staged Interventions for Vocal Fold Paresis and Paralysis: A Proposed Decision Paradigm for Type 1 Thyroplasty.

Journal of voice : official journal of the Voice Foundation·2026
Same journal

Characteristics of Nerve Injury, Regeneration, and Vocal Fold Movement After Recurrent Laryngeal Nerve Electrocautery Injury.

Journal of voice : official journal of the Voice Foundation·2026
Same journal

Effects of Stabilization Exercises on Head Posture, Vocal Efficiency and Pain in Patients with Nonspecific Chronic Neck Pain.

Journal of voice : official journal of the Voice Foundation·2026
Same journal

Correlation Between Age, Gender, and Self-Perceived Voice Handicap in Lebanese Patients With Hoarseness.

Journal of voice : official journal of the Voice Foundation·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

Construction and Characterization of a Novel Vocal Fold Bioreactor
11:11

Construction and Characterization of a Novel Vocal Fold Bioreactor

Published on: August 1, 2014

9.7K

Particulate Matter-Induced Lysosomal Rupture-Mediated Pyroptosis in Human Vocal Fold Fibroblasts.

Hyunsu Choi1, Jooin Bang2, Yaegi Song2

  • 1Clinical Research Institute, Daejeon St. Mary's Hospital, Daejeon, South Korea.

Journal of Voice : Official Journal of the Voice Foundation
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

Particulate matter (PM) exposure causes cell death called pyroptosis in human vocal fold fibroblasts by activating the NLRP3 inflammasome and destabilizing lysosomes. Cathepsin B (CTSB) also plays a role in this PM-induced inflammatory response.

Keywords:
Cathepsin BLaryngitisParticulate matterPyroptosisVocal fold

More Related Videos

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
09:26

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Published on: February 20, 2015

9.5K
Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

2.4K

Related Experiment Videos

Last Updated: Jun 29, 2026

Construction and Characterization of a Novel Vocal Fold Bioreactor
11:11

Construction and Characterization of a Novel Vocal Fold Bioreactor

Published on: August 1, 2014

9.7K
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
09:26

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Published on: February 20, 2015

9.5K
Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

2.4K

Area of Science:

  • Cellular Biology
  • Toxicology
  • Immunology

Background:

  • Particulate matter (PM) is a major air pollutant linked to respiratory diseases.
  • PM exposure is known to trigger NLRP3 inflammasome-mediated pyroptosis.
  • The specific role of cathepsin B (CTSB) in PM-induced pyroptosis remains unclear.

Purpose of the Study:

  • To investigate the involvement of CTSB and NLRP3 inflammasome activation in PM-exposed human vocal fold fibroblasts (hVFFs).

Main Methods:

  • Assessed pyroptotic cell death using LDH release and PI staining.
  • Evaluated NLRP3 inflammasome activation via western blotting and immunofluorescence.
  • Analyzed inflammatory response using ELISA and lysosomal stability with LysoTracker staining.

Main Results:

  • PM exposure induced pyroptosis in hVFFs via NLRP3 inflammasome activation and lysosomal destabilization.
  • Specific inhibitors MCC950 (NLRP3) and CA-074-me (CTSB) suppressed PM-induced pyroptosis and associated inflammatory markers.
  • Results indicate a mechanism involving NLRP3, caspase-1, GSDMD, IL-1β, and CTSB in PM-induced pyroptosis.

Conclusions:

  • Both NLRP3 inflammasome and CTSB are key players in PM-induced pyroptosis in hVFFs.
  • Targeting these pathways may offer therapeutic strategies against PM-related respiratory damage.