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Related Experiment Video

Updated: Mar 22, 2026

The Organoid Reconstitution Assay ORA for the Functional Analysis of Intestinal Stem and Niche Cells

The Organoid Reconstitution Assay ORA for the Functional Analysis of Intestinal Stem and Niche Cells

Published on: November 20, 2017

Organoid Perturbations as Tools to Explore Cellular Function and Plasticity.

Katharina E Kohl^1,2, Georg A Busslinger^1,2

¹1Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria;

Annual Review of Genomics and Human Genetics

|March 20, 2026

Summary

This summary is machine-generated.

Organoids, stem cell-derived models, offer powerful ways to study human biology and disease. Perturbation strategies reveal cellular logic, advancing organoids from descriptive to predictive research tools.

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Last Updated: Mar 22, 2026

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Published on: March 25, 2022

Area of Science:

Biomedical Research
Stem Cell Biology
Tissue Engineering

Background:

Organoids are stem cell-derived models capturing tissue organization, homeostasis, and disease.
They provide physiologically relevant and adaptable systems for studying development, regeneration, and tumor biology.
Organoids offer an experimentally accessible alternative to in vivo studies for human biology.

Purpose of the Study:

To review current organoid perturbation studies.
To highlight methodological advances and applications.
To establish a framework for using organoids as predictive systems.

Main Methods:

Diverse perturbation strategies including genetic edits, pharmacological interventions, and microenvironmental manipulations.
Analysis of cellular and tissue function under controlled experimental conditions.
Review of existing literature on organoid perturbation studies.

Main Results:

Perturbation strategies reveal the molecular logic of cellular and tissue function.
Organoids have advanced the study of development, regeneration, and tumor biology.
Methodological innovations are enabling more complex and predictive organoid models.

Conclusions:

Organoid perturbation studies are crucial for understanding complex biological systems.
Organoids are transitioning from descriptive models to predictive systems.
Future efforts aim to engineer human tissue behavior using organoid platforms.