Comparing Sperm Selection Tools to Improve DNA Integrity

Sperm selection tools are used in assisted reproduction to isolate spermatozoa with the least DNA fragmentation (DF) to optimize fertilization, improve pregnancy outcome and reduce genetic abnormalities in the offspring.

This study aims to compare the sperm DF after three different sperm selection tools were performed on split samples from the neat (unprocessed, control) semen sample. (1) Density gradient centrifugation (DGC). (2) Density gradient centrifugation with swim-up (DGC + swim-up). (3) Microfluidic chip (ZyMot). The primary outcome was the DF of spermatozoa after sperm selection tool as measured by Halosperm test. Scientific time taken to perform the test and the cost of consumables were recorded.

This was a prospective assessor blinded controlled trial between split semen samples conducted at Care Fertility (Australia) in 2024/2025 to compare DF after sperm selection. For each sample, a routine semen analysis was performed, and residual sample was split for processing by sperm selection tools.

Thirty participants consented and underwent the trial. Neat (control) semen: mean sperm DF = 9.5% DGC: mean sperm DF = 4.83% AUD34.11 consumables + 60 min scientific time. DGC+Swim-up: mean sperm DF = 1.8% AUD34.73 consumables + 90 min scientific time. Microfluidic Chip mean sperm DF = 0.06% AUD143.46 consumables + 30 min scientific time.

Microfluidic chip is highly effective at selecting sperm with the least DF and is faster to perform than DGC +/- Swim up.