Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

46.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
46.3K
Spermatogenesis01:41

Spermatogenesis

124.6K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
124.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating real-world uptake of the online Military to Civilian Adjustment and Reintegration Measure among former military members.

Australian journal of psychology·2026
Same author

Indications for Micro-Injection of Sperm in Assisted Reproduction: A Consensus Statement.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same author

Pandemic Recovery Using a COVID-Minimal Cancer Surgery Pathway.

The Annals of thoracic surgery·2020
Same author

Mood and anxiety disorders in Australia and New Zealand's indigenous populations: A systematic review and meta-analysis.

Psychiatry research·2017
Same journal

Determining the Feasibility of a No-Ultrasound Screening Tool for Early Medical Abortion in Australia.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same journal

'All In': Equitable Health Outcomes Require Culturally Safe Care for Everybody.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same journal

Benzylpenicillin Concentrations in Intrapartum Group B Streptococcus Prevention Guidelines; A Systematic Review of the Evidence.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same journal

Retrospective Cohort Study of Foetal Growth Restricted Births After 40 Weeks at an Australian Tertiary Maternity Hospital Between 2018 and 2021.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same journal

Models of Antenatal Care for Rural Indigenous Women: A Systematic Review.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same journal

Dispensing of Antiemetics During Pregnancy in Aotearoa New Zealand, 2005-2020.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
See all related articles

Related Experiment Video

Updated: Mar 27, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

762

Comparing Sperm Selection Tools to Improve DNA Integrity.

Ashleigh Smith1, Sonia Badrak1, Josephine Pinto2

  • 1Consultant (Fertility specialist), Brisbane, Australia.

The Australian & New Zealand Journal of Obstetrics & Gynaecology
|March 26, 2026
PubMed
Summary
This summary is machine-generated.

The microfluidic chip significantly reduces sperm DNA fragmentation (DF) compared to density gradient centrifugation (DGC) methods. This advanced sperm selection tool offers a faster and more effective approach for assisted reproduction, improving outcomes.

More Related Videos

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

3.5K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

762
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

3.5K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K

Area of Science:

  • Reproductive Science
  • Andrology
  • Assisted Reproductive Technology (ART)

Background:

  • Sperm selection tools are crucial in assisted reproduction to minimize sperm DNA fragmentation (DF).
  • Reducing DF optimizes fertilization, enhances pregnancy rates, and decreases genetic risks in offspring.
  • This study evaluates three sperm selection techniques for their efficacy in reducing DF.

Purpose of the Study:

  • To compare sperm DNA fragmentation (DF) levels after using three distinct sperm selection methods: Density Gradient Centrifugation (DGC), DGC with Swim-up, and Microfluidic Chip (ZyMot).
  • To assess the scientific time and consumable costs associated with each sperm selection technique.
  • To determine the most effective sperm selection tool for minimizing DF in assisted reproduction.

Main Methods:

  • A prospective, assessor-blinded controlled trial involving split semen samples from 30 participants.
  • Sperm DNA fragmentation was measured using the Halosperm test post-selection.
  • Routine semen analysis was performed, followed by processing with DGC, DGC + Swim-up, or Microfluidic Chip.

Main Results:

  • Neat (control) semen showed a mean DF of 9.5%.
  • DGC resulted in a mean DF of 4.83% (60 min, AUD34.11).
  • DGC + Swim-up yielded a mean DF of 1.8% (90 min, AUD34.73).
  • Microfluidic Chip achieved a mean DF of 0.06% (30 min, AUD143.46).

Conclusions:

  • The Microfluidic Chip (ZyMot) is highly effective in selecting spermatozoa with minimal DNA fragmentation.
  • This method is significantly faster than DGC and DGC + Swim-up.
  • The Microfluidic Chip presents a superior option for sperm selection in ART, offering reduced DF and improved efficiency.