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Advances in 3D Bioprinting: Materials, Processes, and Emerging Applications.

Subin Antony Jose1, Antonia Evtimow1, Pradeep L Menezes1

  • 1Department of Mechanical Engineering, University of Nevada, Reno, NV 89557, USA.

Micromachines
|March 28, 2026
PubMed
Summary

Three-dimensional (3D) bioprinting advances tissue engineering by precisely depositing cells and biomaterials. This review covers bioinks, printing methods, and applications, paving the way for functional tissues and organs.

Keywords:
3D bioprintingbioinkshydrogel scaffoldsorgan-on-a-chiptissue engineering

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Three-dimensional (3D) bioprinting is a rapidly advancing technology.
  • It enables precise spatial control over living cells, biomaterials, and bioactive molecules.
  • This technology holds significant potential for fabricating functional tissues and organs.

Purpose of the Study:

  • To review recent advancements in 3D bioprinting materials, processes, and applications.
  • To emphasize the integration of bioinks, printing methods, and mechanical design for tissue functionality.
  • To discuss challenges and future trends in bioprinting.

Main Methods:

  • Evaluation of natural and synthetic biomaterials (hydrogels, PEG, PLGA) for biocompatibility, printability, and degradation.
  • Comparison of key bioprinting modalities (extrusion, inkjet, laser-assisted) based on resolution, cell viability, and scalability.
  • Discussion of structural considerations including scaffold architecture, mechanical stability, and biomimetic design.

Main Results:

  • Biomaterials and printing methods are advancing, with ongoing research into their properties and performance.
  • Emerging applications include tissue engineering (bone, cartilage, skin), organ-on-a-chip systems, and patient-specific implants.
  • Key challenges remain in standardization, regulation, ethics, and manufacturing scale-up.

Conclusions:

  • 3D bioprinting represents a paradigm shift in biological construct design and manufacturing.
  • Integration of AI, robotics, multi-material, and 4D bioprinting will drive future advancements.
  • These developments bridge the gap between laboratory research and clinical translation for regenerative medicine.