Three-dimensional organoid screening using a Leica Thunder microscope

Area of Science:

• Biomedical Research
• Microscopy
• 3D Tissue Models

Background:

• 3D organoids provide superior models for studying development, disease, and drug responses compared to 2D cultures.
• High-throughput imaging of complex 3D organoid structures presents significant challenges.
• Advanced imaging techniques are crucial for unlocking the full potential of organoid research.

Purpose of the Study:

• To present a validated method for high-throughput screening of 3D organoids.
• To demonstrate the utility of the Leica Thunder microscope with Computational Clearing for deep 3D imaging.
• To provide a comprehensive protocol for organoid sample preparation, imaging, and analysis.

Main Methods:

• Utilized the Leica Thunder microscope equipped with Computational Clearing technology.
• Developed and optimized a protocol for 3D organoid sample preparation.
• Established image acquisition parameters using LAS X software.
• Implemented FiJi/ImageJ for quantitative analysis of 3D organoid data.

Main Results:

• Successfully demonstrated enhanced deep imaging capabilities for 3D organoids.
• The protocol facilitates efficient and reproducible high-throughput screening.
• Computational Clearing significantly improves image quality and data acquisition depth.

Conclusions:

• The Leica Thunder microscope offers an efficient solution for high-throughput 3D organoid screening.
• This method advances biomedical research, particularly in drug discovery and disease modeling.
• The presented protocol enables robust quantitative analysis of complex 3D tissue models.