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Updated: Apr 7, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

In Vivo Detection of IAA Using IAA Nanosensor.

Benny Jian Rong Sng^1,2, Duc Thinh Khong¹, Thomas K Porter³

¹Disruptive and Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.

Methods in Molecular Biology (Clifton, N.J.)

|April 1, 2026

Summary

This summary is machine-generated.

We developed a novel nanosensor for direct, real-time measurement of the plant hormone indole-3-acetic acid (IAA). This sensor works in green tissues and across plant species, offering a versatile tool for plant science research.

Keywords:

Auxin Corona phase molecular recognition Indole-3-acetic acid Nanosensor Plant stress Single-walled carbon nanotube

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Area of Science:

Plant Biology
Biochemistry
Nanotechnology

Background:

Indole-3-acetic acid (IAA) is a crucial auxin phytohormone regulating plant development, growth, and stress responses.
Accurate and real-time measurement of IAA is essential for understanding these processes.
Existing methods may have limitations in complex plant tissues or across species.

Purpose of the Study:

To present a detailed method and protocol for a novel corona phase molecular recognition-based nanosensor for IAA.
To enable direct and real-time measurement of IAA in various plant species and tissues.
To overcome limitations of existing IAA detection methods, particularly in green tissues.

Main Methods:

Development of a corona phase molecular recognition-based nanosensor for IAA.
Utilizing a near-infrared signal for detection, unaffected by chlorophyll.
Application and validation of the nanosensor across diverse plant species and tissues.

Main Results:

The nanosensor allows for direct and real-time measurement of IAA in plants.
The near-infrared signal enables effective use in green tissues, independent of chlorophyll content.
The nanosensor demonstrates broad applicability across different plant species and all tissue types.

Conclusions:

The developed IAA nanosensor provides a robust and versatile tool for plant hormone research.
This method facilitates non-invasive, real-time monitoring of IAA levels in plants.
The sensor's independence from genetic encoding and chlorophyll interference enhances its utility in diverse plant science applications.