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Updated: Apr 7, 2026
08:57
Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
642
Advances and applications in sequencing-based pathogen surveillance.
Hao Luo1, Yao Wang1, Huiyu Hou1
1Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Abiotech
|April 6, 2026
Summary
Advanced sequencing technologies, including multiplex PCR and AI, offer improved pathogen detection. Innovative primer design and culturomics enhance sensitivity and specificity for global health applications.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Emerging infectious diseases require advanced diagnostic tools.
- Traditional methods face limitations in speed, sensitivity, and scope.
- High-throughput sequencing, especially multiplex PCR, shows promise for pathogen detection.
Purpose of the Study:
- To review advancements in sequencing technologies for pathogen detection.
- To highlight innovative primer design strategies for multiplex PCR.
- To explore the integration of AI in sequencing for diagnostics.
Main Methods:
- Culturomics, metagenomics, and metatranscriptomics for pathogen discovery.
- Development of error-tolerant primer design balancing coverage and specificity.
- Integration of artificial intelligence to enhance sequencing precision and scalability.
Main Results:
- Multiplex PCR offers enhanced sensitivity, specificity, and cost-efficiency in pathogen detection.
- Innovative primer design overcomes limitations like dimerization and bias.
- AI integration enables real-time diagnostics and scalable sequencing.
Conclusions:
- Advances in sequencing technologies and AI provide robust pathogen-detection systems.
- These innovations support global health, food security, and ecological resilience.
- Optimized multiplex PCR and AI integration are key to future diagnostics.
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