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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jul 1, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Transgene-free genome editing in plants.

Yue-Hao Gao1, Qi Lin1, Yu-Jing Wang1

  • 1State Key Laboratory of Tree Genetics and Breeding, School of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.

Abiotech
|June 30, 2026
PubMed
Summary
This summary is machine-generated.

Transgene-free genome editing precisely modifies plant DNA without foreign gene integration. This revolutionary approach enables stable genomic changes, minimizing risks and advancing crop development for agriculture.

Keywords:
Base editingPrime editingRibonucleoprotein complexesTransgene-freemRNA delivery systems

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Breeding by Design for Functional Rice with Genome Editing Technologies
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Breeding by Design for Functional Rice with Genome Editing Technologies

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Last Updated: Jul 1, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

Area of Science:

  • Plant Science
  • Genetics
  • Biotechnology

Background:

  • Conventional genome editing can cause unintended gene fragments, off-target effects, and resistance gene drift.
  • Precise DNA modification is crucial for crop improvement and fundamental plant science.

Purpose of the Study:

  • To review transgene-free genome-editing technologies and their principles.
  • To highlight advancements in DNA-free delivery and its impact on crop development.
  • To discuss future prospects of transgene-free editing in agriculture.

Main Methods:

  • Direct delivery of ribonucleoprotein complexes (RNPs).
  • mRNA delivery systems for transient CRISPR component expression.
  • Base editing (cytosine and adenine base editors - CBEs, ABEs) for single nucleotide substitution.
  • Prime editing for precise modifications using reverse transcriptase templates.

Main Results:

  • Transgene-free methods minimize foreign DNA integration, enabling precise and stable genomic modifications.
  • CRISPR components delivered via RNPs or mRNA avoid stable integration of foreign DNA.
  • Developed crops are considered non-genetically modified due to the absence of integrated foreign DNA.

Conclusions:

  • Transgene-free genome editing offers a revolutionary breakthrough in plant science and agriculture.
  • These technologies minimize risks associated with conventional genome editing.
  • Future developments combining delivery systems with AI optimization show promising agricultural applications.