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Updated: Jan 9, 2026
00:08
A Rapid In Vivo Bioassay for Developmentally Active Enhancers
1.4K
Developmental regulators and additives in promoting genetic transformation and genome editing efficiency
Yi-Xuan Niu1, Qing-Yang Wu1, Shu-Ning Ren1
1State Key Laboratory of Tree Genetics and Breeding, School of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, PR China.
Plant Physiology and Biochemistry : PPB
|December 5, 2025
Summary
Developmental regulators (DRs) boost plant genetic transformation efficiency in recalcitrant species. Combining DRs with additives offers a promising strategy to improve crop yield and quality.
Area of Science:
- Plant biotechnology
- Molecular biology
- Agricultural science
Background:
- Efficient gene delivery is crucial for crop improvement, but most plant species are recalcitrant to genetic transformation.
- Genetic transformation efficiency in higher plants remains below 0.1%.
Purpose of the Study:
- To review the molecular mechanisms and applications of developmental regulators (DRs) in enhancing plant genetic transformation.
- To explore the role of exogenous and endogenous additives in conjunction with DRs.
- To provide perspectives on advancing plant genetic transformation technologies for crop improvement.
Main Methods:
- Systematic review of recent studies on developmental regulators and additives.
- Analysis of molecular mechanisms underlying totipotency and regeneration.
- Examination of applications in recalcitrant plant species.
Main Results:
- Developmental regulators reprogram plant cells, enhancing totipotency and regeneration.
- DRs, especially with additives like peptides and compounds, significantly improve transformation and regeneration efficiency.
- This approach offers a viable strategy for genetically modifying previously recalcitrant plant species.
Conclusions:
- Developmental regulators are key to overcoming low genetic transformation efficiency in many plant species.
- The combined use of DRs and additives presents a powerful tool for plant genetic engineering.
- Further utilization of DRs can advance agricultural and forestry crop improvement for yield and quality.
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