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Updated: May 20, 2026
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
Published on: September 18, 2017
Erythrocyte Integrity, Thymocyte Volume Regulation, and Functional State of the Heart during Ischemia/Reperfusion in
O J Khamidova1,2, N D Oljaev1, D D Fayziev1,2
1Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Republic of Uzbekistan.
Abstract:
In the streptozotocin-nicotinamide model of type 2 diabetes mellitus (T2DM), erythrocytes exhibit increased resistance to osmotic and colloid-osmotic lysis. For thymocytes, cell volume regulation in T2DM was found to be significantly less effective than in the control. Using the Langendorff isolated heart retrograde perfusion model, we demonstrated that reperfusion after 30-min global ischemia leads to a dramatic decrease in HR, a decrease in left-ventricular systolic pressure, left-ventricular developed pressure, and the rate of left-ventricular pressure rise and fall. While the general pattern of heart dynamics during ischemia/reperfusion was preserved in T2DM, post-reperfusion HR was significantly lower than in the control.
