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Published on: November 21, 2023
Defect engineering-mediated OER-enhanced ECL for ultrasensitive detection of E.coli
Yiting Shao1, Xuemei Wang2, Hanxi Liu3
1College of Chemistry and Chemical Engineering, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao University, Qingdao 266071, China; Characteristic Laboratory of Advanced Metal Functional Materials and Processing in Universities of Shandong, School of Mechanical and Electronic Engineering, Qingdao Binhai University, Qingdao 266555, China.
Defect engineering in Ferrocene monocarboxylic acid-modified nickel-based metal-organic frameworks enhances electrochemiluminescence (ECL) for sensitive E. coli detection. This strategy boosts reactive oxygen species generation, improving ECL intensity and biosensor performance.
Area of Science:
- Materials Science
- Electrochemistry
- Nanotechnology
Background:
- The Luminol electrochemiluminescence (ECL) system faces limitations due to slow kinetics and oxygen dependency.
- Developing efficient catalysts for oxygen evolution reaction (OER) is crucial for enhancing ECL performance.
Purpose of the Study:
- To engineer defects in nickel-based metal-organic frameworks (NiBDC MOFs) using Ferrocene monocarboxylic acid (FcCA) to improve OER catalysis.
- To enhance the kinetic process and ECL intensity of the Luminol/H2O system.
- To develop a sensitive biosensor for pathogen detection.
Main Methods:
- Coordinative linkage of FcCA to NiBDC MOFs to create Fc-NiBDC MOFs.
- Investigating defect-induced OER catalysis and reactive oxygen species (ROS) generation.
- Constructing a Luminol-based ECL biosensor immobilized on Fc-NiBDC MOFs.
Main Results:
- Fc-NiBDC MOFs exhibited significantly improved OER performance, leading to a 4-fold increase in Luminol ECL intensity.
- FcCA induced coordination defects and oxygen vacancies, facilitating ROS conversion and concentration.
- An efficient ROS cyclic regeneration mechanism was established through the Fc/Fc+ redox cycle.
- The biosensor achieved a low limit of detection (LOD) of 0.33 CFU mL-1 for E. coli detection.
Conclusions:
- Defect engineering in MOFs offers a novel strategy to boost ECL performance by enhancing ROS generation and cycling.
- The developed Fc-NiBDC MOF platform provides a versatile and efficient system for sensitive pathogen diagnostics.
- This work opens new avenues for MOF-based ECL systems in biosensing applications.
